Fluorescence-immunocytochemistry: simultaneous localization of catecholamines and gonadotropin-releasing hormone

Gonadotropin-releasing hormone and dopamine were identified simultaneously in the same block of tissue from the median eminence of the rat brain. Two distinct bands of dopamine terminals were found in the lateral median eminence: an inner band which overlapped the gonadotropin-releasing hormone terminals and an outer band which appeared juxtaposed to portal capillaries.