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Related Experiment Video

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Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma
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Imaging mass cytometry for high-dimensional tissue profiling in the eye.

Anja Schlecht1,2, Stefaniya Boneva1, Henrike Salie3

  • 1Faculty of Medicine, Eye Center, University of Freiburg, Killianstrasse 5, 79106, Freiburg, Germany.

BMC Ophthalmology
|September 21, 2021
PubMed
Summary

Imaging mass cytometry (IMC) allows detailed analysis of up to 40 markers in single tissue sections. This study demonstrates IMC

Keywords:
IMCImaging mass cytometryconjunctival melanomamulti-dimensional cellular profiling

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Area of Science:

  • Biomedical imaging
  • Ophthalmology
  • Pathology

Background:

  • Imaging mass cytometry (IMC) integrates flow cytometry, mass spectrometry, and laser scanning for high-resolution tissue analysis.
  • IMC utilizes isotope-coupled antibodies for multiplex analysis of up to 40 markers simultaneously, overcoming limitations of traditional methods like immunohistochemistry.

Purpose of the Study:

  • To evaluate the utility of IMC for analyzing formalin-fixed, paraffin-embedded conjunctival tissue.
  • To determine the possibilities, relevance, and limitations of IMC in understanding ocular disease biology and pathology.

Main Methods:

  • An 18-biomarker IMC analysis was performed on conjunctival tissue samples.
  • Standard manufacturer protocols were followed without modification.

Main Results:

  • Successful and interpretable staining was achieved for 12 out of 18 biomarkers.
  • Bioinformatical analysis identified 24 distinct cellular clusters based on marker expression profiles.

Conclusions:

  • IMC provides highly multiplexed imaging of ocular samples at subcellular resolution.
  • IMC is a feasible and innovative method for gaining new insights into ocular disease pathogenesis.
  • Findings are valuable for basic research, drug discovery, and clinical diagnostics in ophthalmology.