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Updated: Oct 19, 2025

Analyzing Mitochondrial Morphology Through Simulation Supervised Learning
Published on: March 3, 2023
Segmentation of Static and Dynamic Atomic-Resolution Microscopy Data Sets with Unsupervised Machine Learning Using
Ning Wang1, Christoph Freysoldt1, Siyuan Zhang1
1Max-Planck-Institut für Eisenforschung GmbH, Max-Planck-Straße 1, 40237Düsseldorf, Germany.
Abstract:
We present an unsupervised machine learning approach for segmentation of static and dynamic atomic-resolution microscopy data sets in the form of images and video sequences. In our approach, we first extract local features via symmetry operations. Subsequent dimension reduction and clustering analysis are performed in feature space to assign pattern labels to each pixel. Furthermore, we propose the stride and upsampling scheme as well as separability analysis to speed up the segmentation process of image sequences. We apply our approach to static atomic-resolution scanning transmission electron microscopy images and video sequences. Our code is released as a python module that can be used as a standalone program or as a plugin to other microscopy packages.
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