Related Experiment Video
Updated: Oct 19, 2025

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
A New 1,5-Disubstituted Triazole DNA Backbone Mimic with Enhanced Polymerase Compatibility
Sven Epple1, Aman Modi1, Ysobel R Baker1
1Chemistry Research Laboratory, University of Oxford, Oxford, OX1 3TA, U.K.
Abstract:
Triazole linkages (TLs) are mimics of the phosphodiester bond in oligonucleotides with applications in synthetic biology and biotechnology. Here we report the RuAAC-catalyzed synthesis of a novel 1,5-disubstituted triazole (TL2) dinucleoside phosphoramidite as well as its incorporation into oligonucleotides and compare its DNA polymerase replication competency with other TL analogues. We demonstrate that TL2 has superior replication kinetics to these analogues and is accurately replicated by polymerases. Derived structure-biocompatibility relationships show that linker length and the orientation of a hydrogen bond acceptor are critical and provide further guidance for the rational design of artificial biocompatible nucleic acid backbones.
Related Concept Videos
Translesion DNA Polymerases
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
The Replisome
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
Proofreading
Errors During Replication are Corrected by the DNA Polymerase...
Homologous Recombination
Nucleic Acid Structure
DNA Structure
DNA...
DNA Topoisomerases
Types and Mechanism of action
Topoisomerases are divided into two main types. ...

