Radiocarbon signature reveals that most springtails depend on carbon from living plants

Terrestrial carbon cycling is largely mediated by soil food webs. Identifying the carbon source for soil animals has been desired to distinguish their roles in carbon cycling, but it is challenging for small invertebrates at low trophic levels because of methodological limitations. Here, we combined radiocarbon (¹⁴C) analysis with stable isotope analyses (¹³C and ¹⁵N) to understand feeding habits of soil microarthropods, especially focusing on springtail (Collembola). Most Collembola species exhibited lower Δ¹⁴C values than litter regardless of their δ¹³C and δ¹⁵N signatures, indicating their dependence on young carbon. In contrast with general patterns across all taxonomic groups, we found a significant negative correlation between δ¹⁵N and Δ¹⁴C values among the edaphic Collembola. This means that the species with higher δ¹⁵N values depend on C from more recent photosynthate, which suggests that soil-dwelling species generally feed on mycorrhizae to obtain root-derived C. Many predatory taxa exhibited higher Δ¹⁴C values than Collembola but lower than litter, indicating non-negligible effects of collembolan feeding habits on the soil food web. Our study demonstrated the usefulness of radiocarbon analysis, which can untangle the confounding factors that change collembolan δ¹⁵N values, clarify animal feeding habits and define the roles of organisms in soil food webs.