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Updated: Oct 19, 2025

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Published on: January 1, 2017
Non-integrating episomal vectors induced pluripotent stem cell line (SDPHi001-A) from a healthy female individual
Jing Shao1, Chunhong Shao1, Yan Jin1
1Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.
Abstract:
Isolated peripheral blood mononuclear cells (PBMCs) from a healthy female were successfully transformed into induced pluripotent stem cells (iPSCs) by non-integrating episomal vectors containing OCT4, SOX2, c-MYC, KLF4, and BCL-XL. The iPSCs displayed a normal karyotype, expressed pluripotency markers and could differentiate into three germ layers in vitro. This iPSC line may be subsequently used for further researches.

