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Related Experiment Video

Updated: Oct 19, 2025

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
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A surrogate cell-based SARS-CoV-2 spike blocking assay.

Wolfgang Schuh1, Lena Baus1, Tobit Steinmetz1

  • 1Division of Molecular Immunology, Department of Internal Medicine 3, Nikolaus-Fiebiger-Zentrum, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany.

European Journal of Immunology
|September 21, 2021
PubMed
Summary
This summary is machine-generated.

A new, affordable SARS-CoV-2 blocking assay (SUBA) was developed to measure antibody levels. This assay can quickly screen antibodies and sera for blocking activity against SARS-CoV-2 variants.

Keywords:
COVID-19SARS-CoV-2Spike proteinSurrogate blocking assayhACE2

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Area of Science:

  • Virology
  • Immunology
  • Assay Development

Background:

  • Monitoring SARS-CoV-2 infection and vaccine efficacy requires assessing neutralizing antibody titers.
  • Existing methods for antibody assessment can be costly and require specialized laboratory facilities.

Purpose of the Study:

  • To develop a rapid, cost-effective surrogate assay for detecting SARS-CoV-2 blocking antibodies.
  • To enable pre-screening of therapeutic antibodies and vaccinated individuals' sera against SARS-CoV-2 variants.

Main Methods:

  • Developed a surrogate SARS-CoV-2 blocking assay (SUBA) using immobilized human ACE2 (hACE2) and human cells expressing SARS-CoV-2 spike protein.
  • Quantified antibody binding to spike protein-expressing cells via crystal violet staining and spectrophotometry.

Main Results:

  • SUBA is a fast, inexpensive assay not requiring high biosafety levels.
  • The assay detects blocking antibodies against native, cell-bound SARS-CoV-2 spike protein.
  • SUBA can be adapted to screen against emerging SARS-CoV-2 variants.

Conclusions:

  • SUBA provides a valuable tool for monitoring SARS-CoV-2 immunity and vaccine effectiveness.
  • The assay's adaptability facilitates rapid assessment of therapeutic antibodies and sera against new variants.