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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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Mass Spectrometry: Overview01:19

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
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Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
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Electrospray Ionization Ion Mobility Mass Spectrometry.

Katie M Kohoutek1, Peter de B Harrington1

  • 1Department of Chemistry and Biochemistry, Ohio University, Athens, OH, USA.

Critical Reviews in Analytical Chemistry
|September 22, 2021
PubMed
Summary
This summary is machine-generated.

Electrospray ionization ion mobility mass spectrometry (ESI-IMS-MS) is a powerful analytical method for analyzing complex molecules. This technique separates isomers and identifies various compounds, with broad applications in pharmaceuticals and environmental science.

Keywords:
Ion mobility mass spectrometryapplications

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Area of Science:

  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Electrospray ionization ion mobility mass spectrometry (ESI-IMS-MS) is an advanced analytical technique.
  • It is used for analyzing complex compounds in the gas phase.

Purpose of the Study:

  • To review the history and development of ESI-IMS-MS.
  • To highlight the diverse applications of ESI-IMS-MS in various scientific fields.

Main Methods:

  • The review synthesizes information on the evolution of ESI-IMS-MS.
  • It covers the principles of isomer separation and structural information acquisition.

Main Results:

  • ESI-IMS-MS effectively separates isomers and provides structural insights.
  • The technique enables quantitative identification of peptides, lipids, carbohydrates, polymers, and metabolites.
  • Applications span pharmaceuticals, environmental analysis, and manufacturing for characterizing drugs, petroleum products, and metal macromolecules.

Conclusions:

  • ESI-IMS-MS is a rapidly advancing technique with significant analytical capabilities.
  • Its versatility makes it valuable across multiple scientific and industrial sectors.