Related Experiment Video

Updated: Oct 19, 2025

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
04:21

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Published on: November 1, 2024

915

[7SK truncation at 128-179 nt suppresses embryonic stem cell proliferation in vitro by downregulating CDC6]

Rui Chen1, Yurong Zhang1, Peng Chen2

  • 1First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, China.

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|September 22, 2021
PubMed
Abstract

Insights

Small nuclear RNA 7SK truncation impairs embryonic stem cell (ESC) proliferation by downregulating CDC6. This finding highlights 7SK

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Embryonic stem cells (ESCs) possess self-renewal and differentiation capabilities.
  • Primordial dwarfism (PD) is a group of rare genetic disorders characterized by severe intrauterine and postnatal growth retardation.
  • Small nuclear noncoding RNA 7SK plays a role in various cellular processes.

Purpose of the Study:

  • To investigate the function of small nuclear noncoding RNA 7SK in ESC proliferation.
  • To evaluate the potential of 7SK as a diagnostic and therapeutic target for primordial dwarfism (PD).

Main Methods:

  • CRISPR/Cas9 gene editing was used to create 7SK deletion mutations in R1 ESCs.
  • Lentivirus-mediated knockdown of cyclin-dependent kinase 9 (CDK9) was performed.
  • Western blotting was employed to analyze protein expression levels, including CDC6.

Main Results:

  • A novel 7SK deletion mutation (128-179 nt) was identified in ESCs, leading to deficient cell proliferation.
  • 7SK truncation significantly reduced the expression of La-related protein 7 (LARP7) and cell division cycle 6 (CDC6).

Conclusions:

  • 7SK truncation at 128-179 nt impairs ESC proliferation by downregulating CDC6.
  • 7SK is a critical regulator of ESC proliferation, mediated by CDK9 activity.
  • 7SK truncation presents a potential target for the early diagnosis and treatment of primordial dwarfism.
Keywords:
7SK truncationcell division cycle 6cyclin-dependent kinase 9embryonic stem cellproliferation

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