Water monitoring by means of digital microscopy identification and classification of microalgae

Marine and freshwater microalgae belong to taxonomically and morphologically diverse groups of organisms spanning many phyla with thousands of species. These organisms play an important role as indicators of water ecosystem conditions since they react quickly and predictably to a broad range of environmental stressors, thus providing early signals of dangerous changes. Traditionally, microscopic analysis has been used to identify and enumerate different types of organisms present within a given environment at a given point in time. However, this approach is both time-consuming and labor intensive, as it relies on manual processing and classification of planktonic organisms present within collected water samples. Furthermore, it requires highly skilled specialists trained to recognize and distinguish one taxa from another on the basis of often subtle morphological differences. Given these restrictions, a considerable amount of effort has been recently funneled into automating different steps of both the sampling and classification processes, making it possible to generate previously unprecedented volumes of plankton image data and obtain an essential database to analyze the composition of plankton assemblages. In this review we report state-of-the-art methods used for automated plankton classification by means of digital microscopy. The computer-microscope system hardware and the image processing techniques used for recognition and classification of planktonic organisms (segmentation, shape feature extraction, pigment signature determination and neural network grouping) will be described. An introduction and overview of the topic, its current state and indications of future directions the field is expected to take will be provided, organizing the review for both experts and researchers new to the field.