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The Mycobacterium tuberculosis sRNA F6 Modifies Expression of Essential Chaperonins, GroEL2 and GroES
Joanna Houghton1, Angela Rodgers2, Graham Rose3
1Faculty of Infectious Tropical Diseases, London School of Hygiene and Tropical medicine, London, United Kingdom.
Microbiology Spectrum
|September 22, 2021
Summary
Mycobacterium tuberculosis small RNA F6 is induced during starvation and infection. Deleting F6 dysregulates essential chaperonins GroEL2 and GroES, impacting bacterial survival.
Area of Science:
- Microbiology
- Molecular Biology
- RNA Biology
Background:
- Tuberculosis remains a significant global health challenge, caused by Mycobacterium tuberculosis.
- Key aspects of M. tuberculosis gene regulation, particularly posttranscriptional control by small RNAs (sRNAs), are not well understood.
Purpose of the Study:
- To investigate the function and regulation of the M. tuberculosis sRNA F6.
- To elucidate the role of F6 in bacterial adaptation and survival during infection.
Main Methods:
- Characterization of F6 expression under starvation conditions and in a mouse infection model.
- Analysis of F6's impact on gene expression, specifically targeting chaperonins GroEL2 and GroES.
- Gene deletion studies to assess F6's role in M. tuberculosis survival.
Main Results:
- F6 expression is dependent on SigF and induced by starvation and infection.
- F6 regulates the expression of essential chaperonins GroEL2 and GroES.
- Deletion of F6 leads to dysregulation of GroEL2/GroES and impairs M. tuberculosis long-term survival.
Conclusions:
- F6 plays a crucial role in M. tuberculosis adaptation to nutrient scarcity and host environments.
- F6-mediated regulation of chaperonins is vital for bacterial persistence within human granulomas.
Keywords:
Mycobacterium tuberculosisWayne model chaperoninshypoxiainfectioninfection modelsmicroarraysnutrient starvationpersistencesmall RNAsmall regulatory RNAMore Related Videos
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