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Updated: Oct 19, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
Published on: July 9, 2021
Assembly of long L-RNA by native RNA ligation
Chen-Hsu Yu1, Adam M Kabza1, Jonathan T Sczepanski1
1Department of Chemistry, Texas A&M University, College Station, TX, USA. jon.sczepanski@chem.tamu.edu.
Abstract:
Due to their intrinsic nuclease resistance, L-oligonucleotides are being increasingly utilized in the development of molecular tools and sensors. Yet, it remains challenging to synthesize long L-oligonucleotides, potential limiting future applications. Herein, we report straightforward and versitile approach to assemble long L-RNAs from two or more shorter fragments using T4 RNA ligase 1. We show that this approach is compatible with the assembly of several classes of functional L-RNA, which we highlight by generating a 124 nt L-RNA biosensor that functions in serum.
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