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A scalable workflow to characterize the human exposome.

Xin Hu1, Douglas I Walker2, Yongliang Liang1

  • 1Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, School of Medicine at Emory University, Atlanta, GA, USA.

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|September 23, 2021
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Summary

Understanding the human exposome is crucial. A new method enables broad environmental chemical analysis in plasma and tissue, advancing exposome research and biomonitoring.

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Area of Science:

  • Environmental health science
  • Analytical chemistry
  • Toxicology

Background:

  • Understanding the human exposome, encompassing all environmental exposures, is vital for public health.
  • Current chemical analysis methods are costly and limited in scope, hindering comprehensive exposome research.
  • A need exists for scalable, cost-effective methods to analyze a wider range of environmental chemicals in biological samples.

Purpose of the Study:

  • To develop and validate a novel, single-step analytical workflow for operationalizing the human exposome.
  • To enable high-throughput quantification of environmental chemicals in human biological matrices.
  • To facilitate population-level exposome research and harmonize biomonitoring efforts.

Main Methods:

  • Development of a single-step express liquid extraction technique.
  • Application of gas chromatography-high-resolution mass spectrometry (GC-HRMS) for chemical analysis.
  • Validation of the method for human plasma (200 µL) and tissue (≤100 mg) samples.

Main Results:

  • The workflow successfully quantifies environmental chemicals in small volumes of human plasma and tissue.
  • High resolution, sensitivity, and selectivity were achieved for mass spectral features.
  • The method allows for exposome epidemiology without prior knowledge of chemical identity.

Conclusions:

  • The developed method operationalizes the human exposome by enabling broad-scale chemical analysis.
  • Its simplicity and efficiency support harmonization of environmental biomonitoring across laboratories.
  • This approach facilitates population-level human exposome research with limited sample volumes.