Related Experiment Video
Updated: Oct 19, 2025
09:16
U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index
Published on: March 28, 2025
354
Tissue and sperm handling before assisted reproductive technology (ART): A systematic review
Rafael Favero Ambar1,2, Marcello M Gava1,2, Milton Ghirelli-Filho1,2
1Urology Department at Centro Universitario Em Saude Do ABC - FMABC, Santo André, Brazil.
Arab Journal of Urology
|September 23, 2021
Summary
This review examines tissue and sperm handling techniques for intracytoplasmic sperm injection (ICSI). It identifies optimal methods to maximize sperm quality and fertilization success in clinical practice.
Area of Science:
- Reproductive Medicine
- Embryology
- Andrology
Background:
- Surgical sperm retrieval is crucial for intracytoplasmic sperm injection (ICSI).
- Optimal handling of retrieved tissue and sperm is essential for successful fertilization.
- Current literature lacks a comprehensive overview of best practices for sperm processing post-retrieval.
Purpose of the Study:
- To systematically review and synthesize techniques for tissue and sperm handling after surgical retrieval for ICSI.
- To identify methods that enhance sperm count, vitality, and function.
- To discuss optimal combination strategies for clinical application.
Main Methods:
- A systematic literature search was conducted in PubMed and Google Scholar.
- Studies were selected based on modified Preferred Reporting Items for Systemic Reviews and Meta-Analyses (PRISMA) guidelines.
- The review focused on tissue handling and sperm selection techniques for ICSI.
Main Results:
- 42 articles were included in the analysis.
- Common techniques for sample handling and sperm selection were identified.
- The use of chemical compounds to improve sperm motility and fertilization rates was investigated.
Conclusions:
- The ideal sperm handling protocol ensures high sperm count, vitality, and function without adverse effects.
- This review details common and effective techniques for sperm processing.
- Evidence-based combination strategies are discussed for various clinical scenarios.
