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MicroRNA 452 regulates GTF2E1 expression in colorectal cancer cells

Ji Su Mo1, Soo Cheon Chae

  • 1Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk 54538, Republic of Korea chaesc@wku.ac.kr.

Journal of Genetics
|September 23, 2021
PubMed

Insights

MicroRNA 452 (MIR452) is upregulated in colorectal cancer (CRC) and downregulates General Transcription Factor IIE Subunit 1 (GTF2E1) expression. This suggests MIR452 plays a role in CRC pathogenesis by affecting GTF2E1 levels.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • MicroRNAs regulate gene expression and are implicated in human disease pathogenesis.
  • MicroRNA 452 (MIR452) is specifically upregulated in early-stage human colorectal cancer (CRC).

Purpose of the Study:

  • To investigate the biological role of MIR452 in colorectal cancer.
  • To determine the relationship between MIR452 and General Transcription Factor IIE Subunit 1 (GTF2E1) expression in CRC.

Main Methods:

  • Luciferase reporter assay to confirm MIR452's effect on GTF2E1.
  • Quantitative RT-PCR (qRT-PCR) and western blotting to evaluate expression levels.
  • Transfection of CRC cells with MIR452.

Main Results:

  • MIR452 directly downregulates GTF2E1 transcripts.
  • MIR452 transfection decreased both mRNA and protein levels of GTF2E1 in CRC cells.
  • GTF2E1 protein expression was reduced in CRC tissues compared to adjacent non-tumour tissues.

Conclusions:

  • MIR452 may regulate CRC-related gene transcription by downregulating GTF2E1.
  • MIR452's role in CRC pathogenesis warrants further investigation.

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