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Draft Genome Sequences of 142 Mycobacterium avium subsp. paratuberculosis Strains Isolated from Naturally Infected

Cyril Conde1, Maxime Branger1, Thierry Cochard1

  • 1INRAE, Université de Tours, ISP, Nouzilly, France.

Microbiology Resource Announcements
|September 23, 2021
PubMed

Insights

This study presents the genome sequences of 142 Mycobacterium avium subsp. paratuberculosis strains from French dairy cattle. These findings offer insights into the pathogen causing Johne

Area of Science:

  • Veterinary Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease, a chronic intestinal infection in ruminants.
  • Johne's disease significantly impacts the dairy industry worldwide.
  • Understanding MAP's genetic diversity is crucial for disease control.

Purpose of the Study:

  • To provide annotated draft genome sequences for 142 MAP strains.
  • To analyze the genomic landscape of MAP strains isolated from French dairy cattle.
  • To establish a genomic resource for future epidemiological and evolutionary studies.

Main Methods:

  • Whole-genome sequencing of 142 MAP isolates using Illumina technology.
  • Bioinformatic analysis for genome annotation and characterization.
  • Isolation of strains from dairy cattle in France (2014-2018).

Main Results:

  • Successfully generated annotated draft genome sequences for all 142 MAP strains.
  • The study provides a comprehensive genomic dataset of MAP from a specific geographic region and time frame.
  • Initial analyses reveal genetic variations within the studied MAP population.

Conclusions:

  • The genome sequences provide a valuable resource for understanding MAP epidemiology and pathogenesis.
  • This genomic data can aid in developing improved diagnostics and control strategies for Johne's disease.
  • Further research can leverage these sequences to investigate MAP evolution and transmission dynamics.

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