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Updated: Oct 19, 2025

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Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
Published on: March 13, 2020
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Determination of wheat moisture using terahertz spectroscopy combined with the tabu search algorithm
Yin Shen1,2, Chunjiang Zhao1,2, Bin Li3,2
1College of Engineering and Technology, Southwest University, Chongqing 400715, China.
Analytical Methods : Advancing Methods and Applications
|September 23, 2021
Summary
Terahertz spectroscopy combined with the Tabu Search (TS) algorithm accurately detects wheat moisture content. This rapid method, utilizing spectral data, offers a reliable approach for grain analysis and storage.
Area of Science:
- Agricultural Science
- Spectroscopy
- Chemometrics
Background:
- Accurate wheat moisture detection is crucial for grain storage and classification.
- Traditional methods can be time-consuming and less precise.
Purpose of the Study:
- To develop a rapid and accurate method for quantitative analysis of moisture content in wheat.
- To evaluate the effectiveness of terahertz spectroscopy combined with chemometric algorithms for this purpose.
Main Methods:
- Terahertz (THz) time-domain spectroscopy was applied to 240 wheat samples.
- Spectral data underwent pretreatment (S-G, MSC, SNV) and variable selection using Tabu Search (TS).
- Quantitative models were built and validated using Partial Least Squares (PLS) regression.
Main Results:
- High correlation coefficients for calibration (Rc=0.9522) and prediction (Rp=0.9531) were achieved.
- Low root mean square errors for calibration (RMSEC=0.4730) and prediction (RMSEP=0.5396) indicate model accuracy.
- The S-G + MSC + TS + PLS model demonstrated effective prediction of wheat moisture content.
Conclusions:
- Terahertz time-domain spectroscopy, coupled with the TS algorithm, provides an accurate quantitative analysis of wheat moisture.
- This integrated approach offers a rapid and reliable method for detecting moisture in wheat samples, benefiting grain management.
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