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Influenza Virus Propagation in Embryonated Chicken Eggs
Published on: March 19, 2015
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Virulence attitude estimation of Pasteurella multocida isolates in embryonated chicken eggs
Azhar G Shalaby1, Neveen R Bakry1, Azza S El-Demerdash2
1Agriculture Research Center, National Lab for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki, Egypt.
Archives of Microbiology
|September 23, 2021
Summary
Fowl cholera, caused by Pasteurella multocida type A, significantly impacts chickens and ducks in Egypt, showing resistance to multiple drugs. Multiplex PCR is effective for rapid diagnosis.
Area of Science:
- Veterinary Microbiology
- Avian Pathology
- Antimicrobial Resistance
Background:
- Fowl cholera, a significant avian disease, poses economic threats to poultry farming.
- Prevalence and characteristics of Pasteurella multocida in Egyptian poultry require investigation.
Purpose of the Study:
- To isolate and identify Pasteurella multocida from chickens and ducks in Sharkia Governorate, Egypt.
- To determine the antimicrobial susceptibility and pathogenicity of isolated strains.
- To evaluate multiplex PCR as a diagnostic tool for fowl cholera.
Main Methods:
- Bacteriological isolation and identification of Pasteurella multocida.
- Multiplex polymerase chain reaction (PCR) for capsular typing.
- Disk diffusion assay and Minimum Inhibitory Concentration (MIC) determination for antimicrobial susceptibility.
- Pathogenicity studies in embryonated chicken eggs (ECE).
Main Results:
- Pasteurella multocida was isolated from 10% of chickens and 6% of ducks, with infection linked to age and breed.
- All isolates were identified as type A strains using multiplex PCR.
- High susceptibility to amikacin, doxycycline, chloramphenicol, and neomycin was observed, with doxycycline being most effective.
- Twenty-five percent of isolates exhibited multidrug resistance.
- Pathogenicity varied among isolates, with higher concentrations causing embryo mortality and characteristic lesions.
Conclusions:
- Pasteurella multocida serogroup A is a significant cause of fowl cholera in mature chickens, leading to economic losses.
- Multiplex PCR is a valuable technique for the rapid and accurate diagnosis of fowl cholera.
- Antimicrobial resistance is a growing concern, necessitating careful treatment strategies.
