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Published on: September 26, 2013
Influence of interleukin 17 A and 17 F polymorphisms in keratoconus
Isabela Bronchtein Gomes1, Christiane Maria Ayo1, Alessandro Garcia Lopes2
1Faculty of Medicine of São José do Rio Preto (FAMERP), Department of Molecular Biology, Laboratory of Immunogenetics, São José do Rio Preto, SP, Brazil.
Background:
Until a few years ago, keratoconus was defined as a noninflammatory degenerative disease. However, recent studies have shown that the altered balance between inflammatory cytokines, proteases, and protease inhibitors, as well as free radicals and oxidants, have a crucial role in the pathogenesis of this disease. The aim of this study is to investigate whether interleukin 17 A G197A (rs2275913) and interleukin 17 F T7488C (rs763780) polymorphisms are associated with keratoconus in patients from a population of the northwestern region of the State of São Paulo, Brazil.
Methods And Results:
35 patients and 61 controls were enrolled. Genotyping of interleukin 17 A G197A and interleukin 17 F T7488C polymorphisms was carried out using the polymerase chain reaction-restriction fragment length polymorphism technique. Statistical analyses were conducted using the chi-square test, and an odds ratio with a 95% confidence interval was also calculated to evaluate the association between polymorphisms and disease. Evaluating interleukin 17 F T7488C, we found that the TT genotype is associated as a risk factor for keratoconus (P = 0.04; OR = 3.01; CI 1.11-8.14). As for evaluating interleukin 17 A G197A, the allele and genotype frequencies between patients and controls were compared and no statistically significant differences were found.
Conclusions:
Our data showed that the interleukin 17 F T7488C polymorphisms may exert an influence in keratoconus.
