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Related Experiment Video

Updated: Oct 19, 2025

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

Defining variant-resistant epitopes targeted by SARS-CoV-2 antibodies: A global consortium study.

Kathryn M Hastie¹, Haoyang Li¹, Daniel Bedinger²

¹Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA.

Science (New York, N.Y.)

|September 23, 2021

Summary

Antibody treatments are crucial for fighting COVID-19. Researchers mapped the SARS-CoV-2 spike protein, finding key antibodies effective against evolving variants.

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Published on: June 14, 2022

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Last Updated: Oct 19, 2025

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Engineering Antiviral Agents via Surface Plasmon Resonance

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

Area of Science:

Virology
Immunology
Structural Biology

Background:

Antibody therapeutics and vaccines are vital for managing COVID-19 severity and fatalities.
Mutations in SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) can reduce the effectiveness of antibody defenses during transmission and zoonotic events.

Purpose of the Study:

To map the epitope landscape of the SARS-CoV-2 spike protein.
To structurally characterize antibody communities targeting the receptor binding domain (RBD).
To assess the impact of viral mutations on antibody efficacy.

Main Methods:

Formation of an international consortium for epitope mapping.
Structural illustration of RBD-directed antibody communities.
Pseudovirion-based neutralization assays to evaluate antibody function against mutated spike proteins.

Main Results:

Defined seven distinct RBD-directed antibody communities with unique binding sites and competition patterns.
Identified specific spike mutations, both individual and clustered in variants, that impact antibody neutralization.
Demonstrated that key classes of RBD-targeted antibodies retain neutralization activity against emerging SARS-CoV-2 variants.

Conclusions:

Established a framework for selecting effective antibody treatment cocktails.
Provided insights into how viral variants may influence the efficacy of antibody therapies.
Highlighted the importance of continued surveillance of viral mutations for therapeutic development.