Free amino acids of rat astrocytes in primary culture: changes during cell maturation

The concentrations of free amino acids were analysed in cultured primary astrocytes during cell maturation and in the starting material, i.e. the cerebral hemispheres of newborn rats. Taurine was the most abundant amino acid in all samples, the content of glutamine being comparable only in immature astrocytes (7 days in culture). The intracellular levels of most amino acids significantly decreased during the first 2 weeks in culture, remaining fairly stable during the third week.