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Published on: December 10, 2013
Human adenoviruses in paediatric patients with respiratory tract infections in Beijing, China
Yiman Huang1, Chao Wang1, Fenlian Ma1
1NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
Insights
Human adenovirus (HAdV) causes respiratory infections in children. This study identified HAdV-B3 and HAdV-B7 as dominant strains in Beijing, with a concerning rise in recombinant HAdV-C CBJ113.
Area of Science:
- Virology
- Epidemiology
- Pediatrics
Background:
- Human adenovirus (HAdV) is a significant cause of pediatric respiratory tract infections (RTIs).
- Genetic mutations and recombination in HAdV can alter its pathogenicity and transmission dynamics.
- Understanding the epidemiology and genetic diversity of HAdV is crucial for managing pediatric RTIs.
Purpose of the Study:
- To investigate the epidemiology and genotypic diversity of human adenovirus (HAdV) in hospitalized children with RTIs in Beijing, China.
- To identify the predominant HAdV species and strains circulating in this population.
- To analyze the association between viral load and clinical symptoms.
Main Methods:
- Collection of nasopharyngeal aspirates from hospitalized children with RTIs between April 2018 and March 2019.
- Detection of HAdVs using quantitative real-time PCR.
- Phylogenetic analysis of the hexon gene to determine genotypic diversity and identify strains.
Main Results:
- Human adenovirus (HAdV) was detected in 5.72% (90/1572) of samples, with peak detection rates in November and July.
- Co-infections with other respiratory viruses, including human respiratory syncytial virus and human rhinovirus, were common (61.11%).
- HAdV-B (63.64%) and HAdV-C (27.27%) were the main epidemic species. Phylogenetic analysis revealed the circulation of recombinant HAdV-C strain CBJ113, alongside HAdV-B3 and HAdV-B7.
Conclusions:
- Human adenovirus-B3 (HAdV-B3) and Human adenovirus-B7 (HAdV-B7) are the primary epidemic strains in Beijing.
- The recombinant Human adenovirus-C (HAdV-C) strain CBJ113 shows an emerging epidemic trend.
- High viral load in HAdV infections is associated with increased fever and elevated white blood cell count in children.
Background:
Human adenoviruse (HAdV) is a major pathogen of paediatric respiratory tract infections (RTIs). Mutation or recombination of HAdV genes may cause changes in its pathogenicity and transmission. We described the epidemiology and genotypic diversity of HAdV in hospitalized children with RTIs in Beijing, China.
Methods:
Nasopharyngeal aspirates were collected from hospitalized children with RTIs from April 2018 to March 2019. HAdVs were detected by a quantitative real-time PCR, and the hexon gene was used for phylogenetic analysis.
Results:
Among 1572 samples, 90 (5.72%) were HAdV-positive. The HAdV detection rate was highest in November and July. Among HAdV-positive children, 61.11% (55/90) were co-infected with other respiratory viruses, the most common of which were human respiratory syncytial virus and human rhinovirus. The main diagnosis was bronchopneumonia, most patient have cough and fever. Children with a high viral load were more likely to have a high fever (P = 0.041) and elevated WBC count (P = 0.000). Of 55 HAdV-positive specimens, HAdV-B (63.64%), HAdV-C (27.27%), and HAdV-E (9.09%) were main epidemic species. Phylogenetic analysis indicated that hexon sequences of three samples were on the same branch with the recombinant HAdV strain (CBJ113), which was circulating in Beijing since 2016.
Conclusion:
The HAdV-B3 and HAdV-B7 are the main epidemic strains in Beijing, and the recombinant HAdV-C strain CBJ113 has formed an epidemic trend.
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