High resolution banding of chronic myeloid leukemia chromosomes

Bone marrow aspirates from 29 patients with chronic myeloid leukemia were studied using the methotrexate synchronization culture method. Successful cytogenetic preparations exhibiting long and well banded chromosomes were obtained from all of them. The standard t(9;22) was seen in 23 patients, four had variant translocations, and two were Ph-negative. Of the four patients with variant translocations, one had a simple translocation in which the missing segment of chromosome #22 was translocated onto the short arm of chromosome #9. The remaining three patients had complex translocations. The first involved chromosomes #11, #19, and #22, the second involved chromosomes #9, #11, and #22, and the third involved chromosomes #9, #14, and #22. Karyotypic abnormalities in addition to the Ph chromosome were seen in four patients: in three these changes developed during the chronic phase and in one during the blastic phase. Using Q-, R-, and G-banding techniques, we found that the breakpoint on chromosome #22 is just below the centromere, namely in band 22q11.2 and on chromosome #9 in band 9q34.1. The standard translocation, therefore, can be written as t(9;22)(q34.1;q11.2). Furthermore, the breakpoint on 22q appeared to be identical in all cases with standard as well as the variant translocations. Our results show that the methotrexate synchronization method permits consistent high resolution banding of CML chromosomes, and support the concept that there is no difference in the amount of material translocated from 22q in different patients.