Related Experiment Video

Updated: Oct 19, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

5.8K

LncRNA CDKN2B-AS1 contributes to glioma development by regulating the miR-199a-5p/DDR1 axis

Jiachao Lu1, Yuanjun Chen1, Liangbao Wen1

  • 1Department of Neurosurgery, The first affiliated hospital of Guangxi Medical University, Nanning City, Guangxi Zhuang Autonomous Region, P.R. China.

The Journal of Gene Medicine
|September 24, 2021
PubMed
Abstract

Insights

Cyclin-dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1) promotes glioma development by interacting with miR-199a-5p and DDR1. This finding suggests CDKN2B-AS1 as a potential therapeutic target for glioma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Glioma is a common primary brain tumor with complex molecular underpinnings.
  • The role of long non-coding RNAs (lncRNAs) like CDKN2B-AS1 in glioma pathogenesis is increasingly recognized but not fully elucidated.

Purpose of the Study:

  • To investigate the functional role of CDKN2B-AS1 in glioma.
  • To elucidate the molecular mechanism by which CDKN2B-AS1 influences glioma progression.
  • To assess the therapeutic potential of targeting CDKN2B-AS1 in glioma.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure CDKN2B-AS1 expression in glioma tissues and cell lines.
  • Loss-of-function studies using short hairpin RNA (sh-CDKN2B-AS1) to assess effects on cell proliferation, invasion, migration, and apoptosis.
  • Bioinformatics analysis and luciferase reporter assays to determine interactions between CDKN2B-AS1, miR-199a-5p, and DDR1.
  • In vivo xenograft models to validate findings in a living organism.

Main Results:

  • CDKN2B-AS1 is significantly upregulated in glioma tissues and cell lines.
  • Knockdown of CDKN2B-AS1 inhibits glioma cell proliferation, invasion, and migration, while promoting apoptosis.
  • CDKN2B-AS1 positively regulates DDR1 expression by sponging miR-199a-5p, and this axis drives glioma progression.
  • CDKN2B-AS1 knockdown suppressed tumor growth in a mouse xenograft model.

Conclusions:

  • CDKN2B-AS1 plays a crucial role in promoting glioma development.
  • The miR-199a-5p/DDR1 axis is a key mediator of CDKN2B-AS1's oncogenic function in glioma.
  • CDKN2B-AS1 represents a promising therapeutic target for glioma treatment.
Keywords:
CDKN2B-AS1DDR1gliomamiR-199a-5p

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.6K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.1K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.3K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.3K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.4K