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Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
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A ROS-responsive fluorescent probe detecting experimental colitis by functional polymeric nanoparticles.

Yanbing Wang1, Xiaolei Wang2, Yingni Lv2

  • 1Shanghai Key Laboratory of New Drug Design, East China University of Science and Technology, Shanghai 200237, China; Department of Pharmaceutics, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.

International Journal of Pharmaceutics
|September 24, 2021
PubMed
Summary

This study developed a novel nanoparticle system using totalROX to detect oxidative stress in inflammatory bowel disease (IBD). The system accurately measures colitis severity, offering a promising tool for diagnosis.

Keywords:
Inflammatory bowel diseaseOral nanoparticle delivery systemROS-responsive fluorescence probepH-responsive

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Gastroenterology

Background:

  • Oxidative stress is crucial in inflammatory bowel disease (IBD) pathogenesis.
  • Current diagnostic methods for colitis lack sensitivity in assessing oxidative stress levels.

Purpose of the Study:

  • To develop and evaluate a nanoparticle-based fluorescent probe for detecting colitis severity.
  • To investigate the potential of totalROX and oral nanoparticle delivery for monitoring oxidative stress in IBD.

Main Methods:

  • A pH-responsive nanoparticle system incorporating totalROX, Eudragit S100, hyaluronic acid, and chitosan was synthesized.
  • An experimental colitis mouse model was induced using dextran sodium sulfate.
  • Nanoparticle targeting, inflammation assessment, and correlation with oxidative stress markers were evaluated.

Main Results:

  • The nanoparticles demonstrated efficient colon targeting and stability.
  • A strong positive correlation was found between fluorescence intensity (Ox670) and myeloperoxidase activity (R² = 0.97) and histopathological scores (R² = 0.98).
  • TotalROX effectively measured reactive oxygen species (ROS) in inflamed Caco-2 cells.

Conclusions:

  • Oral administration of totalROX-loaded nanoparticles provides a sensitive method for evaluating colitis degree in vivo.
  • This approach represents a novel ROS-responsive fluorescent probe for IBD diagnosis and fluorescence-guided colonoscopy.