A novel belowground in-situ gas labeling approach: CH₄ oxidation in deep peat using passive diffusion chambers and...

In-vitro incubation of environmental samples is a common approach to estimate CH₄ oxidation potential. Here we developed and verified an in-situ method utilizing passive diffusion chambers (PDC, silicone tubes) to deliver ¹³C-labeled CH₄ into peat for the determination of the CH₄ oxidation potential based on ¹³C excess of CO₂. To target CH₄ oxidation under semi-aerobic and anaerobic conditions, we installed 20 PDCs (30 ml volume) below the water table in profiles from 35-cm to 2-m depths of a peatland in north-eastern Sweden in July 2017 using a peat auger. ¹³C-labeled CH₄ was injected into PDCs through tubing twice during 12 days (day 0 and 6) and samples were collected at days 1, 3, 6, 8 and 11. Background (non-labeled) δ¹³C of CO₂ ranged from -7.3 (35 cm) to +5.7‰ (200 cm) with depth. These δ¹³C values rose to +110 and + 204‰ after the CH₄ injection. The estimated CH₄-derived C in CO₂ was the lowest at the bottom of the profile (0.3 μmol L^-1), whereas the maximum was at 100 cm (6.1 μmol L^-1) at five days after the second labeling. This corresponded to 1.5-7.2% of the total CH₄ pool to be oxidized, depending on depth. This novel approach with belowground in-situ ¹³C labeling of gases demonstrated the suitability of tracing the transformations of these gases in soil depth by PDCs and for the first time verified the in-situ occurrence of a deep-peat CH₄ oxidation.