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Updated: Oct 19, 2025

Intratracheal Administration of Dry Powder Formulation in Mice
Published on: July 25, 2020
Dry powder formulation for pulmonary infections: Ciprofloxacin loaded in chitosan sub-micron particles generated by
Beatriz Arauzo1, M Pilar Lobera1, Antonio Monzon2
1Institute of Nanoscience and Materials of Aragon (INMA) CSIC-Universidad de Zaragoza, Department of Chemical and Environmental Engineering, University of Zaragoza, Campus Río Ebro-Edificio I+D, C/ Mariano Esquillor S/N, 50018 Zaragoza, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, 28029 Madrid, Spain.
Abstract:
Electrospray was used as a one-step technique to generate inhalable ciprofloxacin-loaded chitosan sub-micron particles with potential use in the treatment of pulmonary infections. The effect of operating parameters was studied and the preparation method optimized. The final sizes of ciprofloxacin-loaded particles were 386.1 ± 248.5 nm and 501.1 ± 276.3 nm for high and low molecular weight chitosan, respectively. The high surface charge of the particles formed, around +45 mV, enhances their mucoadhesive properties. The particles were biocompatible with alveolar cell line (A549), and showed a high antimicrobial activity against two of the most common respiratory pathogens Staphylococcus aureus and Pseudomonas aeruginosa.

