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A rapid, simple method for isolation of viable microfilariae.

M Van Hoegaerden, B Ivanoff

    The American Journal of Tropical Medicine and Hygiene
    |January 1, 1986
    PubMed
    Summary

    A new method efficiently purifies Loa loa microfilariae from blood using Percoll gradients and filtration. This technique ensures high purity, viability, and motility, aiding research on parasitic diseases.

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    Area of Science:

    • Parasitology
    • Tropical Medicine
    • Biotechnology

    Background:

    • Loa loa microfilariae are causative agents of onchocerciasis, a neglected tropical disease.
    • Accurate quantification and study of microfilariae require pure, viable parasite populations.
    • Existing purification methods can be time-consuming and may affect parasite motility.

    Purpose of the Study:

    • To develop a rapid and efficient method for purifying Loa loa microfilariae from blood.
    • To ensure the isolated microfilariae retain their viability and motility.
    • To provide a scalable method suitable for large blood volumes.

    Main Methods:

    • Utilizes a discontinuous Percoll gradient for microfilariae separation.
    • Employs filtration on cellulose ester membranes for final purification.
    • The method is designed for simplicity and minimal equipment requirements.

    Main Results:

    • Achieves nearly 100% purity and viability of Loa loa microfilariae.
    • Isolated microfilariae remain motile for at least 20 days.
    • Typical recovery rates from blood samples are around 90%.

    Conclusions:

    • This Percoll gradient and filtration method offers a highly efficient way to purify Loa loa microfilariae.
    • The technique preserves parasite viability and long-term motility, crucial for experimental studies.
    • The method's simplicity and scalability make it suitable for processing large blood volumes and potentially isolating other microfilariae species, such as Mansonella perstans.

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