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Updated: Oct 19, 2025

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
Published on: May 12, 2018
Studies of SMOOTHENED Activation in Cell-Free and Reconstituted Systems
Isaac B Nelson1, Benjamin R Myers2
1Departments of Oncological Sciences, Biochemistry, and Bioengineering, University of Utah School of Medicine, Salt Lake City, UT, USA.
Abstract:
Much of our current understanding of Hedgehog signal transduction derives from studies involving intact cells and organisms. Here we describe the use of cell-free and reconstituted systems to study a key step in Hedgehog signal transduction: the activation of SMOOTHENED by membrane lipids. These methods can be adapted to study other steps in Hedgehog signal transduction, particularly those that occur at the membrane.

