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Updated: Oct 19, 2025

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Published on: June 9, 2017
Measuring Smoothened (SMO)-Mediated Activation of the Gi Protein
1Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Abstract:
The oncoprotein Smoothened (SMO) can transduce the Hedgehog signal from the tumor suppressor Patched-1 (PTCH1) to glioma-associated-oncogene (Gli) transcription factors. Previous studies have shown that SMO is a G-protein-coupled receptor (GPCR) of the Frizzled-class (class-F) that can activate Gi family of heterotrimeric G proteins. Here, we describe [35S]-GTPγS assay using SMO cell membranes and purified Gi protein to measure the level of Gi protein activation following the activation of SMO by agonists.
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