Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Structure01:23

RNA Structure

75.3K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
75.3K
Experimental RNAi02:15

Experimental RNAi

6.5K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K
Types of RNA01:23

Types of RNA

69.6K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
69.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a size-separation technique to isolate Caenorhabditis elegans embryos using mesh filters.

PloS one·2025
Same author

Faculty rewards from course-based undergraduate research experiences (CURE) in biochemistry.

Journal of microbiology & biology education·2025
Same author

Effect of Sequence on the Interactions of Divalent Cations with M-Box Riboswitches from <i>Mycobacterium tuberculosis</i> and <i>Bacillus subtilis</i>.

Biochemistry·2021
Same author

Effect of dC → d(m<sup>5</sup>C) substitutions on the folding of intramolecular triplexes with mixed TAT and C<sup>+</sup>GC base triplets.

Biochimie·2017
Same author

Effect of Mutations on the Binding of Kanamycin-B to RNA Hairpins Derived from the Mycobacterium tuberculosis Ribosomal A-Site.

Biochemistry·2015
Same author

Effect of helix stability on the formation of loop-loop complexes.

Biochemistry·2012
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
See all related articles

Related Experiment Video

Updated: Oct 19, 2025

Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example
12:44

Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example

Published on: December 3, 2014

54.1K

RNA electroelution: Comparing two electroeluter models.

Amber N Rogers1, Maya K Mastronardo1, Tsion G Mekonnen2

  • 1Molecular Biology, Biochemistry & Bioinformatics Program, Towson University, Towson, MD, 21252, USA.

Analytical Biochemistry
|September 25, 2021
PubMed
Summary
This summary is machine-generated.

Researchers compared two electroeluters for purifying RNA from polyacrylamide gels. Both devices effectively recovered comparable amounts of purified RNA, ensuring consistent results for molecular biology applications.

Keywords:
ElectroelutionElectrophoresisGel elutionPAGEPolyacrylamideRNA purification

More Related Videos

Practical Aspects of Sample Preparation and Setup of 1H R1&#961; Relaxation Dispersion Experiments of RNA
08:17

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Published on: July 9, 2021

4.9K
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

8.3K

Related Experiment Videos

Last Updated: Oct 19, 2025

Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example
12:44

Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example

Published on: December 3, 2014

54.1K
Practical Aspects of Sample Preparation and Setup of 1H R1&#961; Relaxation Dispersion Experiments of RNA
08:17

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Published on: July 9, 2021

4.9K
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

8.3K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Purified RNA is essential for various molecular biology techniques.
  • Electroelution from polyacrylamide gels is a common RNA purification method.
  • Discontinuation of electroeluter models necessitates evaluating alternatives.

Purpose of the Study:

  • To compare the RNA recovery efficiency of two electroeluter models with different chamber designs.
  • To assess the suitability of alternative electroeluters for RNA purification.

Main Methods:

  • RNA purification using electroelution from polyacrylamide gel slices.
  • Comparison of RNA yield and purity between two distinct electroeluter devices.

Main Results:

  • Both electroeluters demonstrated effective RNA recovery.
  • No significant difference in purified RNA yield was observed between the two models.
  • Both devices are suitable for obtaining purified RNA for downstream applications.

Conclusions:

  • The choice of electroeluter model does not significantly impact RNA recovery efficiency.
  • Researchers can confidently use either of the tested electroeluters for RNA purification.
  • This study provides valuable data for researchers selecting RNA purification equipment.