Cocaine Detection by a Laser-Induced Immunofluorometric Biosensor.

Area of Science:

• Analytical Chemistry
• Biotechnology
• Forensic Science

Background:

• Drug trafficking poses significant public health risks, necessitating advanced detection methods.
• Current methods for detecting drug smuggling at borders face challenges in speed and sensitivity.
• Illegal drug routes via borders, harbors, and airports are primary supply channels.

Purpose of the Study:

• To develop a highly sensitive and rapid immunosensor for cocaine detection.
• To address the challenge of timely detection of drug traces in smuggling prevention.
• To provide a near-continuous monitoring solution for cocaine at points of entry.

Main Methods:

• Utilized a monolithic affinity column with immobilized hapten and fluorescently labeled antibodies.
• Employed laser-induced fluorescence detection coupled with a Peltier-cooled CMOS camera.
• Integrated high-precision syringe pumps and microfluidic devices for liquid handling.

Main Results:

• Achieved a limit of detection of 7 parts per trillion (ppt) or 23 picomolar (pM) for cocaine.
• Demonstrated a rapid response time of 90 seconds and a total assay time under 3 minutes.
• Successfully detected 300 picograms (pg) of cocaine using surface wipe sampling.

Conclusions:

• The developed immunosensor is among the most sensitive and fastest cocaine detectors available.
• The technology offers a promising solution for near-continuous analyte measurement in drug smuggling interdiction.
• This advanced biosensor can significantly enhance public health protection by improving drug interdiction capabilities.