Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simplified Sample Preparation and Lateral Flow Immunoassay for the Detection of Plant Viruses.

Biosensors·2026
Same author

Corundum Particles as Trypsin Carrier for Efficient Protein Digestion.

Biotech (Basel (Switzerland))·2026
Same author

Cost-Effective Method for Full-Length Sequencing of Monoclonal Antibodies from Hybridoma Cells.

Antibodies (Basel, Switzerland)·2025
Same author

Comparative simulation analysis of photonic ultrasound sensors based on silicon waveguides.

Scientific reports·2025
Same author

Challenges and Insights in Absolute Quantification of Recombinant Therapeutic Antibodies by Mass Spectrometry: An Introductory Review.

Antibodies (Basel, Switzerland)·2025
Same author

Drone-Based Localization of Hazardous Chemicals by Passive Smart Dust.

Sensors (Basel, Switzerland)·2024

Related Experiment Video

Updated: Oct 19, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

34.0K

Cocaine Detection by a Laser-Induced Immunofluorometric Biosensor.

Martin Paul1, Robert Tannenberg1, Georg Tscheuschner1

  • 1Division 1.5 Protein Analysis, Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Strasse 11, 12489 Berlin, Germany.

Biosensors
|September 25, 2021
PubMed
Summary

A new immunosensor detects cocaine with high sensitivity and speed. This device uses a unique affinity column and laser-induced fluorescence for rapid drug smuggling detection at borders and ports.

Keywords:
flow injection assayfluorescence microscopehigh-speedlaser-induced fluorescence detector (LIF)low-costmicrofluidic systemsmonoclonal antibodymonolithic columnonline detectionsecurity

More Related Videos

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
06:58

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

9.8K
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

10.8K

Related Experiment Videos

Last Updated: Oct 19, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

34.0K
Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
06:58

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

9.8K
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

10.8K

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Forensic Science

Background:

  • Drug trafficking poses significant public health risks, necessitating advanced detection methods.
  • Current methods for detecting drug smuggling at borders face challenges in speed and sensitivity.
  • Illegal drug routes via borders, harbors, and airports are primary supply channels.

Purpose of the Study:

  • To develop a highly sensitive and rapid immunosensor for cocaine detection.
  • To address the challenge of timely detection of drug traces in smuggling prevention.
  • To provide a near-continuous monitoring solution for cocaine at points of entry.

Main Methods:

  • Utilized a monolithic affinity column with immobilized hapten and fluorescently labeled antibodies.
  • Employed laser-induced fluorescence detection coupled with a Peltier-cooled CMOS camera.
  • Integrated high-precision syringe pumps and microfluidic devices for liquid handling.

Main Results:

  • Achieved a limit of detection of 7 parts per trillion (ppt) or 23 picomolar (pM) for cocaine.
  • Demonstrated a rapid response time of 90 seconds and a total assay time under 3 minutes.
  • Successfully detected 300 picograms (pg) of cocaine using surface wipe sampling.

Conclusions:

  • The developed immunosensor is among the most sensitive and fastest cocaine detectors available.
  • The technology offers a promising solution for near-continuous analyte measurement in drug smuggling interdiction.
  • This advanced biosensor can significantly enhance public health protection by improving drug interdiction capabilities.