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Rutin Exerts Cytotoxic and Senescence-Inducing Properties in Human Melanoma Cells

Iulia Pinzaru1,2, Raul Chioibas3, Iasmina Marcovici1,2

  • 1Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, "Victor Babeș" University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania.

Toxics
|September 26, 2021
PubMed

Insights

Rutin (RUT), a natural compound, shows potential against malignant melanoma by reducing cancer cell viability and inducing senescence. Further research is needed to understand its anti-melanoma mechanisms.

Area of Science:

  • Oncology
  • Dermatology
  • Pharmacology

Background:

  • Malignant melanoma is an aggressive skin cancer with limited advanced treatment options.
  • Natural compounds are explored for their anticancer properties in melanoma research.
  • Rutin (RUT) exhibits various skin-beneficial activities, but its anti-melanoma effects are underexplored.

Purpose of the Study:

  • To evaluate the cytotoxic potential of Rutin (RUT) against human melanoma cell lines.
  • To assess RUT's impact on cell viability, morphology, nuclear features, and senescence.
  • To investigate RUT as a potential natural chemotherapeutic agent for melanoma.

Main Methods:

  • In vitro assessment of RUT's cytotoxicity on RPMI-7951 and SK-MEL-28 melanoma cell lines.
  • Evaluation of cell viability, morphology, nuclear changes, and senescence induction.
  • Determination of IC50 values and expression of senescent-associated beta-galactosidase (SA-β-gal).

Main Results:

  • RUT demonstrated a dose-dependent decrease in melanoma cell viability.
  • Calculated IC50 values were 64.49 ± 13.27 µM for RPMI-7951 and 47.44 ± 2.41 µM for SK-MEL-28.
  • RUT induced apoptotic features and senescence, evidenced by increased SA-β-gal expression in SK-MEL-28 cells.

Conclusions:

  • Rutin exhibits in vitro cytotoxic and senescence-inducing effects against human melanoma cells.
  • RUT presents potential as a natural agent for melanoma treatment.
  • Further studies are necessary to elucidate the precise mechanisms of RUT's anti-melanoma activity.
Keywords:
cell viabilitycellular senescencehuman melanoma cellsrutin

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