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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
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A potential diagnostic marker for osteosarcoma: hsa_circ_0005721
Ziyin Han1, Zufang Mou, Yulong Jing
1Department of Traumatic Orthopedics, Yantaishan Hospital of Yantai, Yantai, China.
Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology
|September 26, 2021
Summary
Circular RNA hsa_circ_0005721 is elevated in osteosarcoma tissues and plasma, promoting cancer progression. This finding suggests hsa_circ_0005721 holds potential as a diagnostic biomarker for osteosarcoma.
Area of Science:
- Oncology
- Molecular Biology
- Biomarker Discovery
Background:
- Osteosarcoma is a primary bone malignancy with significant morbidity.
- Novel biomarkers are crucial for early diagnosis and improved prognosis of osteosarcoma.
Purpose of the Study:
- To investigate the expression of hsa_circ_0005721 in osteosarcoma.
- To evaluate the clinical significance of hsa_circ_0005721 as a diagnostic marker for osteosarcoma.
Main Methods:
- Quantitative real-time polymerase chain reaction (qRT-PCR) to detect hsa_circ_0005721 expression in tissues and plasma.
- In vitro assays (colony formation, EdU) to assess the role of hsa_circ_0005721 in osteosarcoma cell proliferation.
- Kaplan-Meier analysis for survival and receiver operating characteristic (ROC) curves for diagnostic capacity.
Main Results:
- hsa_circ_0005721 was significantly upregulated in osteosarcoma tissues and cell lines.
- Higher hsa_circ_0005721 levels correlated with poorer overall survival in osteosarcoma patients.
- Downregulation of hsa_circ_0005721 suppressed osteosarcoma cell proliferation.
- Elevated plasma hsa_circ_0005721 levels distinguished osteosarcoma patients from healthy controls and benign bone tumor patients.
Conclusions:
- hsa_circ_0005721 functions as an oncogene, promoting osteosarcoma progression.
- hsa_circ_0005721 is a potential diagnostic biomarker for osteosarcoma.
