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Second-generation sequencing assistance in the diagnosis of Pasteurella multocida empyema: A case report
Bin Liu1, Peng Huang2, Shuangbo Liu2
1First Division, Department of Respiratory and Critical Care Medicine, Zhuzhou Hospital, Affiliated to Xiangya School of Medicine, Central South University/Zhuzhou Central Hospital, Zhuzhou Hunan 412007, China. robin0336@126.com.
Abstract:
Pasteurella multocida empyema is rare and easy to be misdiagnosed. An 81-year-old male patient showed symptoms with cough, sputum, and fever for 3 days. Community-acquired pneumonia was diagnosed firstly. After anti-infection treatment, the patient was still in fever. Chest radiography showed pleural effusion, closed thoracic drainage was performed and the reddish-brown fluid was drained out. The second-generation sequencing was performed on pleural fluid and Pasteurella multocida was detected. Pasteurella multocida has strict requirements for growth conditions and it difficult to cultivate. The application of second-generation sequencing is helpful to diagnose the pathogen rapidly.
Insights
A rare case of Pasteurella multocida empyema was diagnosed in an elderly patient. Second-generation sequencing rapidly identified the pathogen, aiding in timely diagnosis and treatment.
Area of Science:
- Medicine
- Microbiology
- Infectious Diseases
Background:
- Pasteurella multocida empyema is an uncommon condition.
- It is often misdiagnosed, leading to delayed treatment.
Observation:
- An 81-year-old male presented with symptoms mimicking community-acquired pneumonia.
- Despite initial antibiotic treatment, the patient remained febrile.
- Chest radiography revealed pleural effusion, and drainage yielded reddish-brown fluid.
Findings:
- Second-generation sequencing of pleural fluid identified Pasteurella multocida.
- Pasteurella multocida has stringent growth requirements, making cultivation difficult.
Implications:
- Rapid pathogen identification is crucial for effective treatment of rare empyema cases.
- Second-generation sequencing offers a valuable tool for diagnosing difficult-to-cultivate pathogens like Pasteurella multocida.
