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In Vitro Antimicrobial Activity Evaluation of a Novel Fitostimoline® Plus Spray Formulation
Maria Domenica Falciglia1, Roberta Palladino1, Barbara Maglione1
1Farmaceutici Damor S.p.a, Via E. Scaglione 27, Napoli, Italy.
Abstract:
Wound contaminants are the main cause of healing delay and infection in both chronic and acute wounds; for this reason, the microbial infection management in wound healing is one of the most important components for an effective standard of care. The wound contaminants are most likely to originate from the environment and from the surrounding skin lesion, and to date, the most frequent bacteria isolated are Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae. In order to counteract and control these contaminants, the standard care includes topical antiseptic agents. The most commonly used include iodine-releasing agents (e.g., povidone-iodine), hydrogen peroxide, and polyhexanide. This study aims to investigate the in vitro antibacterial activity of a novel topical spray (Fitostimoline® Plus spray) based on 0.1% polyhexanide and Rigenase® against S. aureus, P. aeruginosa, K. pneumoniae, and the combination of S. aureus and K. pneumoniae. The in vitro antimicrobial activity of Fitostimoline® Plus spray was evaluated by the agar disk diffusion assay, quantitative suspension test, and quantitative carrier test, demonstrating that Fitostimoline® Plus spray is able to kill 99.9% bacteria. These results support the microbiological characterization of Fitostimoline® Plus spray confirming the antibacterial activity of polyhexanide (PHMB).
Insights
This study shows Fitostimoline® Plus spray effectively kills 99.9% of common wound bacteria, including Staphylococcus aureus and Pseudomonas aeruginosa. This supports its use for managing microbial infections in wound healing.
Area of Science:
- Microbiology
- Wound Care
- Antimicrobial Research
Background:
- Wound contaminants delay healing and cause infection in acute and chronic wounds.
- Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae are common wound pathogens.
- Effective microbial infection management is crucial for standard wound care.
Purpose of the Study:
- To investigate the in vitro antibacterial activity of Fitostimoline® Plus spray.
- To evaluate the efficacy of a novel topical spray containing 0.1% polyhexanide and Rigenase®.
- To assess the spray's activity against key wound bacteria: S. aureus, P. aeruginosa, K. pneumoniae.
Main Methods:
- Agar disk diffusion assay.
- Quantitative suspension test.
- Quantitative carrier test.
Main Results:
- Fitostimoline® Plus spray demonstrated significant in vitro antibacterial activity.
- The spray was effective against Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae.
- The study confirmed that Fitostimoline® Plus spray kills 99.9% of tested bacteria.
Conclusions:
- Fitostimoline® Plus spray exhibits potent in vitro antimicrobial properties.
- The results support the microbiological profile of Fitostimoline® Plus spray.
- The antibacterial action is attributed to its active component, polyhexanide (PHMB).
