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Related Experiment Video

Updated: Oct 19, 2025

Patterning of Embryonic Stem Cells Using the Bio Flip Chip
05:25

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On-chip multiplexed single-cell patterning and controllable intracellular delivery.

Zaizai Dong1,2, Yanli Jiao3, Bingteng Xie4

  • 1School of Biological Science and Medical Engineering, Beihang University, 100083 Beijing, China.

Microsystems & Nanoengineering
|September 27, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a 3D electroporation platform for efficient, safe single-cell macromolecule delivery. The vacuum-assisted system significantly improves transfection efficiency for applications in cell manipulation and cancer therapy.

Keywords:
BionanoelectronicsNanofabrication and nanopatterningNanostructures

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Nanotechnology

Background:

  • Conventional electroporation methods struggle with macromolecule delivery efficiency, cell damage, and uniformity.
  • Limitations hinder effective in vitro and in vivo applications.

Purpose of the Study:

  • To develop a novel 3D electroporation platform for efficient and safe single-cell intracellular delivery.
  • To overcome limitations of existing electroporation techniques.

Main Methods:

  • Fabrication of a pyramid pit micropore array chip using silicon wet-etching.
  • Integration of a controllable vacuum system for single-cell trapping.
  • Low-voltage electroporation for intracellular delivery of various molecules.

Main Results:

  • Achieved significantly higher transfection efficiency for oligonucleotides and plasmid DNA compared to benchmark methods.
  • Demonstrated controllable drug delivery dosage by adjusting voltage.
  • Successfully knocked out p62 and CXCR7 genes in tumor cells using CRISPR-Cas9, inhibiting cellular activity.

Conclusions:

  • The vacuum-assisted micropore array platform offers a simple, efficient, and high-throughput method for intracellular delivery.
  • This technology facilitates on-chip cell manipulation, intracellular investigation, and potential cancer therapy applications.