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Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability

Gabriela Verónica Díaz1, Romina Olga Coniglio1, Clara Inés Chungara1

  • 1Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones "María Ebe Reca" CONICET. Facultad de Ciencias Exactas, Químicas y Naturales. Universidad Nacional de Misiones. Ruta, Posadas, Misiones, Argentina.

Mycology
|September 27, 2021
PubMed

Insights

This study identified Aspergillus niger LBM 134, a fungus from the Argentinian rainforest, as a potent cellulase producer. This discovery offers a promising microorganism for safe biotechnological processes involving cellulosic biomass conversion.

Area of Science:

  • Mycology
  • Biotechnology
  • Enzymology

Background:

  • Aspergillus section Nigri are known for producing hydrolytic enzymes like cellulases.
  • Cellulases are crucial for cellulose conversion in various industrial applications.
  • Identifying novel cellulolytic fungal isolates is vital for safe and efficient biotechnological processes.

Purpose of the Study:

  • To characterize the cellulolytic potential of Aspergillus sp. LBM 134.
  • To identify the isolated fungus using a polyphasic approach.
  • To assess the suitability of the isolate for industrial cellulosic biomass processing.

Main Methods:

  • Isolation of Aspergillus sp. LBM 134 from the Paranaense rainforest.
  • Identification of the isolate using a polyphasic approach.
  • Evaluation of cellulolytic activity via Congo red and fluorescence plate assays for carboxymethyl cellulase, β-glucosidase, and cellobiohydrolase.

Main Results:

  • The isolate was identified as Aspergillus niger.
  • Positive results were observed for carboxymethyl cellulase, β-glucosidase, and cellobiohydrolase activities.
  • Aspergillus niger LBM 134 demonstrated significant cellulolytic potential.

Conclusions:

  • Aspergillus niger LBM 134 is a promising candidate for industrial applications.
  • The identified strain can be utilized for efficient cellulosic biomass processing.
  • This finding supports the bio-prospecting of novel fungal strains for biotechnological advancements.
Keywords:
Nigricellulasesfluorescence assaysmacromorphologymicromorphologymolecular markers

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