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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
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Related Experiment Video

Updated: Oct 19, 2025

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
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Atomically Thin Bilayer Janus Membranes for Cryo-electron Microscopy.

Liming Zheng1, Nan Liu2, Ying Liu3,4

  • 1Center for Nanochemistry, Beijing Science and Engineering Center for Nanocarbons, Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

ACS Nano
|September 27, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel bilayer Janus graphene membrane for cryo-electron microscopy (cryo-EM). This advanced material improves specimen preparation, enabling higher resolution imaging of biomolecules and materials.

Keywords:
Janusbilayer graphenecryo-EMmechanical robustnear-atomic resolution

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Area of Science:

  • Materials Science
  • Biophysics
  • Electron Microscopy

Background:

  • Cryo-electron microscopy (cryo-EM) is crucial for analyzing beam-sensitive samples.
  • Specimen preparation challenges limit high-resolution cryo-EM analysis.
  • A robust supporting film is needed for desirable sample preparation.

Purpose of the Study:

  • To develop an improved supporting film for cryo-EM.
  • To enhance the resolution and stability of cryo-EM imaging.
  • To enable atomic-resolution characterization of biomolecules and materials.

Main Methods:

  • Fabrication of a bilayer Janus graphene membrane.
  • Functionalization of the top graphene layer for molecular interaction.
  • Maintenance of an intact bottom graphene layer for mechanical support.
  • Application of the membrane in cryo-electron microscopy specimen preparation.

Main Results:

  • The bilayer Janus membrane provides an ultraclean and atomically thin support.
  • The membrane minimizes noise and specimen motion during cryo-EM imaging.
  • Atomic-resolution imaging of surface functional groups was achieved.
  • Direct imaging of lithium dendrites and near-atomic resolution reconstruction of macromolecules were demonstrated.

Conclusions:

  • The bilayer Janus graphene membrane design is a promising support for high-resolution cryo-EM.
  • This material addresses key limitations in cryo-EM specimen preparation.
  • The developed membrane facilitates advanced imaging of diverse samples.