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Updated: Oct 19, 2025
Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling
Published on: October 14, 2021
Selected transient receptor potential channel genes' expression in peripheral blood mononuclear cells of multiple
Murat Çakır1, Hikmet Saçmacı2, Seda Sabah-Özcan3
1Department of Physiology, Faculty of Medicine, 162338University of Yozgat Bozok, Yozgat, Turkey.
Abstract:
Transient receptor potential channels have responsibilities in many cellular processes such as cytokine production, cell differentiation, and cytotoxicity by affecting intracellular cation levels or intracellular signal pathways. Multiple sclerosis is a chronic autoimmune central nervous system (CNS) disease caused by environmental and genetic factors. In this study, we aim to investigate TRPV1-TRPV4, TRPM2, TRPM4, TRPM7, TRPC6, and TRPA1 mRNA expression levels, which are associated with the inflammatory process, in the peripheral blood mononuclear cells (PBMCs) of relapsing-remitting multiple sclerosis (RRMS) patients. Thirty-five healthy controls and age-gender matched thirty patients with RRMS were involved in the study. TRPC6, TRPA1, TRPM2, TRPM4, TRPM7, TRPV1, TRPV2, TRPV3, and TRPV4 PBMCs mRNA expression levels were determined by qPCR. In the present study, the TRPC6, TRPM7, TRPV1, TRPV3, and TRPV4 mRNA expressions of RRMS patients in PBMCs decreased at a significant level compared to the healthy control group (p = .000, p = .000, p = .044, p = .000, p = .004, respectively). The decreased expression of TRPC6, TRPM7, TRPV1, TRPV3, and TRPV4 in PBMCs may be associated with the pathogenesis of MS. Further studies are required to understand the mechanism of the relation between these TRP channels and MS and other autoimmune diseases.
Insights
This study found decreased expression of specific Transient Receptor Potential (TRP) channels, including TRPC6, TRPM7, TRPV1, TRPV3, and TRPV4, in the peripheral blood mononuclear cells of multiple sclerosis patients, suggesting a role in disease pathogenesis.
Area of Science:
- Neuroimmunology
- Ion Channel Biology
Background:
- Transient Receptor Potential (TRP) channels are crucial for cellular functions, including immune responses.
- Multiple Sclerosis (MS) is a chronic autoimmune disease affecting the central nervous system (CNS).
Purpose of the Study:
- To investigate the mRNA expression levels of specific TRP channels (TRPV1-TRPV4, TRPM2, TRPM4, TRPM7, TRPC6, TRPA1) in patients with relapsing-remitting MS (RRMS).
- To explore the association between TRP channel expression and the inflammatory processes in RRMS.
Main Methods:
- Quantitative PCR (qPCR) was used to measure mRNA expression levels of TRP channels in peripheral blood mononuclear cells (PBMCs).
- A cohort of 30 RRMS patients and 35 age-gender matched healthy controls was analyzed.
Main Results:
- Significant decreases in TRPC6, TRPM7, TRPV1, TRPV3, and TRPV4 mRNA expression were observed in RRMS patients compared to healthy controls (p < .05 for all).
- No significant changes were reported for TRPA1, TRPM2, TRPM4, TRPV2.
Conclusions:
- The reduced expression of TRPC6, TRPM7, TRPV1, TRPV3, and TRPV4 in PBMCs may contribute to the pathogenesis of MS.
- Further research is warranted to elucidate the precise mechanisms linking these TRP channels to MS and other autoimmune conditions.
