Akt Phosphorylation Orchestrates T11TS Mediated Cell Cycle Arrest in Glioma Cells
Sagar Acharya1, Sirshendu Chatterjee2, Suhnrita Chaudhuri3
1Department of Zoology, Vidyasagar University, Paschim Medinipur, Midnapore, India.
Abstract:
The novel anti-neoplastic glycopeptide T11TS retards glioma both in in-vitro clinical samples and in-vivo models. This study investigates the correlation between altering the glioma microenvironment with glioma arrest and death. Flow cytometry, immunoblotting, ELISA, and co-immunoprecipitation were employed to investigate glioma cell arrest and death. Results include a decline in phosphorylation of Akt and attenuation of p21 phosphorylation (Thr145,Ser146) and disassociation of p-Akt-Mdm2 and p-Akt-BAD facilitating death by Akt>BAD. T11TS influence phosphorylation patterns in two focal axes Akt>p21 and Akt>Mdm2>p53. The current article provides crucial insight in deciphering the mechanism of T11TS induced glioma cell arrest and death.
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