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A Simplistic Single-Step Method for Preparing Biomimetic Nanoparticles from Endogenous Biomaterials.

Aaron S Schwartz-Duval1,2, Rachele Wen1,2, Indrajit Srivastava1,2

  • 1Department of Bioengineering, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801, United States.

ACS Applied Materials & Interfaces
|September 27, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel, single-step method for creating protein-functionalized gold nanoparticles at room temperature using nicotinamide adenine dinucleotide (NADH). This efficient process avoids harsh conditions, preserving biomolecule integrity for enhanced nanoparticle applications.

Keywords:
NADHendogenous biomaterialsgold nanoparticlesgreen chemistryplasmonics

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Area of Science:

  • Nanotechnology
  • Biomaterials Science
  • Biomedical Engineering

Background:

  • Current methods for functionalizing gold nanoparticles often involve multi-step processes with synthetic molecules, heating, or pH changes.
  • These conventional methods can damage biomolecules, leading to product loss and altered functionality.
  • Utilizing complex biomaterials like cell membranes offers advanced targeting and immune response modulation for nanoparticles.

Purpose of the Study:

  • To develop a single-step, room-temperature method for synthesizing gold nanoparticles functionalized with proteins and whole cell membranes.
  • To preserve the integrity and functionality of biomolecules during nanoparticle synthesis.
  • To offer an improved alternative to existing multistep functionalization strategies.

Main Methods:

  • Gold nanoparticles were synthesized in a single step at room temperature using nicotinamide adenine dinucleotide (NADH).
  • Functionalization was achieved using individual proteins (e.g., FBS) and whole cell membranes (e.g., B16F10 cells).
  • Characterization involved spectroscopy, molecular modeling, electron microscopy, light scattering, and gel electrophoresis.

Main Results:

  • The single-step synthesis successfully produced protein- and cell membrane-functionalized gold nanoparticles.
  • The process maintained biomolecule integrity without requiring additional purification steps.
  • Compared to conventional methods, the synthesized nanoparticles demonstrated superior cellular internalization in vitro.

Conclusions:

  • This novel method provides an efficient and gentle approach for creating functionalized gold nanoparticles.
  • The room-temperature, single-step synthesis preserves biomolecule integrity, offering potential advantages for therapeutic and targeting applications.
  • The process's reliance on endogenous biomaterials and enhanced internalization suggests significant potential for future biomedical applications.