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Chitosan-microencapsulated rhEGF in promoting wound healing
Hsin-Chung Tsai1,2, Christine Sheng3, Le-Shin Chang4
1Department of Surgery, Taichung Hospital, Ministry of Health and Welfare, Taichung, Taiwan.
Journal of Wound Care
|September 27, 2021
Summary
This study shows that a novel chitosan-encapsulated recombinant human epidermal growth factor (rhEGF) spray significantly accelerates wound healing and promotes mature collagen synthesis in rats compared to conventional dressings.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing Research
Background:
- Chitosan and epidermal growth factor (EGF) are known to enhance wound healing.
- Effective topical delivery of rhEGF remains a challenge.
Purpose of the Study:
- To investigate the wound healing efficacy of a novel spray solution containing chitosan-encapsulated recombinant human EGF (rhEGF).
- To evaluate the impact of this rhEGF spray on collagen synthesis and tissue regeneration.
Main Methods:
- Full-thickness wounds were created on Wistar rats.
- Rats were treated with hydrogel, wet dressing, foam, rhEGF spray, or rhEGF spray plus foam.
- Histological evaluations were performed on day 14.
Main Results:
- The rhEGF spray demonstrated the greatest wound size reduction by day 14.
- rhEGF spray significantly increased mature Type I collagen synthesis compared to conventional dressings.
- Regenerated tissue in rhEGF spray groups showed alignment with normal skin, with visible epidermis, dermis, and hair follicles.
Conclusions:
- Chitosan-encapsulated rhEGF nanoparticles overcome topical delivery challenges for rhEGF.
- The novel rhEGF topical spray solution exhibits significant therapeutic potential for wound healing.
