Inhibition of nitrite-oxidizing bacteria in automatic recycling PN/ANAMMOX under mainstream conditions

At present, sustainable and stable partial nitrification has not been widely achieved in the mainstream PN/ANAMMOX process. Here, the feasibility of sustainable and stable partial nitrification was demonstrated in automatic recycling PN/ANAMMOX reactor under mainstream conditions using both simulation and experimental methods. Stable nitrite accumulation in the aerobic zone could be achieved via regulating dissolved oxygen (DO) concentrations and sludge retention time (SRT). The DO concentrations required for the repression of nitrite-oxidizing bacteria (NOB) were lower at longer SRTs. The DO concentrations and SRTs required for NOB repression were lower at lower temperatures. However, NOB repression was diminished by a persistent low DO and short SRT under mainstream conditions. With the introduction of automatic recycling, sustainable and stable partial nitrification was achieved. Effluent recycling could limit the nitrite-nitrogen required for NOB growth. Collectively, effluent recycling may serve as a feasible and useful strategy for NOB inhibition during the PN/ANAMMOX process.