Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic Landscape of Congenital Cataracts in a Swiss Cohort: Addressing Diagnostic Oversights in Nance-Horan Syndrome.

Biomedicines·2025
Same author

Estradiol Downregulates MicroRNA-193a to Mediate Its Anti-Mitogenic Actions on Human Coronary Artery Smooth Muscle Cell Growth.

Cells·2025
Same author

Estradiol Downregulates MicroRNA-193a to Mediate Its Angiogenic Actions.

Cells·2025
Same author

Novel Genetic Variants and Clinical Profiles in Peters Anomaly Spectrum Disorders.

International journal of molecular sciences·2025
Same author

Retinal Dystrophy Associated with Homozygous Variants in <i>NRL</i>.

Genes·2025
Same author

17β-Estradiol Abrogates TNF-α-Induced Human Brain Vascular Pericyte Migration by Downregulating miR-638 via ER-β.

International journal of molecular sciences·2024

Related Experiment Video

Updated: Oct 18, 2025

Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
10:09

Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons

Published on: January 23, 2020

7.1K

Estradiol Inhibits Human Brain Vascular Pericyte Migration Activity: A Functional and Transcriptomic Analysis.

Lisa Kurmann1, Michal Okoniewski2, Raghvendra K Dubey1,3

  • 1Department of Reproductive Endocrinology, University Hospital Zurich, 8952 Schlieren, Switzerland.

Cells
|September 28, 2021
PubMed
Summary

Estradiol, a form of estrogen, protects the blood-brain barrier (BBB) by regulating pericyte activity. This finding offers new insights into stroke prevention and treatment strategies for women.

Keywords:
COVID-19MAPKTNFαblood–brain barrierestrogen receptorinflammationstroke

More Related Videos

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
06:18

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause

Published on: August 13, 2019

12.4K
Ovariectomy and 17&#946;-estradiol Replacement in Rats and Mice: A Visual Demonstration
06:51

Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration

Published on: June 7, 2012

58.1K

Related Experiment Videos

Last Updated: Oct 18, 2025

Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
10:09

Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons

Published on: January 23, 2020

7.1K
An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
06:18

An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause

Published on: August 13, 2019

12.4K
Ovariectomy and 17&#946;-estradiol Replacement in Rats and Mice: A Visual Demonstration
06:51

Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration

Published on: June 7, 2012

58.1K

Area of Science:

  • Neuroscience
  • Endocrinology
  • Vascular Biology

Background:

  • Stroke is a leading cause of mortality in women, with blood-brain barrier (BBB) disruption playing a key role in its pathophysiology.
  • Estrogen's protective vascular effects are known, but its specific impact on BBB integrity and stroke remains unclear.
  • Pericytes are crucial for BBB integrity, and their regulation by estradiol is hypothesized to influence BBB function.

Purpose of the Study:

  • To investigate the impact of estradiol on human brain vascular pericyte (HBVP) functions relevant to BBB integrity.
  • To determine the specific estrogen receptors (ERs) involved in estradiol's effects on pericytes.
  • To elucidate the intracellular mechanisms mediating estradiol's influence on pericyte migration and BBB function.

Main Methods:

  • HBVPs were treated with estradiol, and cell migration and proliferation were assessed.
  • The effects of estradiol on tumor necrosis factor-alpha (TNFα)-induced pericyte migration were evaluated using specific ER agonists and antagonists.
  • Intracellular signaling pathways (AKT and MAPK) were analyzed, and transendothelial electrical resistance (TEER) was measured to assess BBB function.

Main Results:

  • Estradiol inhibited basal and TNFα-induced pericyte migration, an effect mediated by ER-α and ER-β.
  • Estradiol reduced TNFα-induced MAPK phosphorylation, which was critical for abrogating migratory actions.
  • Estradiol enhanced BBB barrier function in co-cultures of pericytes and endothelial cells and modulated gene expression related to cell migration.

Conclusions:

  • Estradiol modulates pericyte activity, specifically inhibiting migration via ER-α and ER-β signaling pathways.
  • These modulatory effects contribute to improved endothelial barrier integrity, suggesting a protective role against BBB disruption.
  • Findings provide the first evidence of estradiol's direct impact on pericyte function, offering potential therapeutic targets for stroke.