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Genomic Investigation of Methicillin-Resistant Staphylococcus aureus ST113 Strains Isolated from Tertiary Care

Nimat Ullah1, Hamza Arshad Dar1, Kanwal Naz1

  • 1Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Islamabad 44000, Pakistan.

Antibiotics (Basel, Switzerland)
|September 28, 2021
PubMed

Insights

This study analyzes two Methicillin-resistant Staphylococcus aureus (MRSA) strains from Pakistan, revealing the emerging ST113 clone. Findings detail genetic diversity and antibiotic resistance, crucial for tracking MRSA spread.

Area of Science:

  • Microbiology
  • Genomics
  • Epidemiology

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) is a significant multidrug-resistant pathogen.
  • Limited epidemiological data exists for MRSA isolates in Pakistan.
  • Emerging MRSA clones necessitate enhanced surveillance.

Purpose of the Study:

  • To conduct a whole-genome comparative analysis of two MRSA strains from Pakistan.
  • To understand the genetic diversity, sequence type (ST), and gene distribution of MRSA isolates.
  • To characterize the emerging ST113 MRSA clone in Pakistan.

Main Methods:

  • Whole-genome sequencing and comparative genomic analysis.
  • Identification of sequence types (STs), SCCmec types, plasmids, and prophage sequences.
  • Analysis of antibiotic resistance and virulence genes.

Main Results:

  • Two MRSA strains, P10 and R46, were identified as ST113, harboring SCCmec type IV.
  • Specific antibiotic resistance genes (e.g., aph(3')-IIIa, aad(6), tet(K), mupA) and a fusidic acid resistance mutation were found.
  • Phylogenetic analysis indicated ST113 is a single-locus variant of ST8.

Conclusions:

  • The study characterizes the emerging ST113 MRSA clone in Pakistan.
  • Findings provide crucial genomic data for MRSA surveillance and comparative analysis.
  • The sequenced strains serve as valuable references for global ST113 and Pakistani MRSA studies.
Keywords:
ST113antibiotic-resistancecomparative genome analysismethicillin-resistant Staphylococcus aureusmulti-locus sequence typewhole-genome sequencing

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