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Transcriptomic Changes Following Partial Depletion of CENP-E in Normal Human Fibroblasts
Danilo Cilluffo1,2, Roberta Flavia Chiavetta1, Serena Bivona1,3
1Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, 90128 Palermo, Italy.
Genes
|September 28, 2021
Summary
Reducing CENP-E protein levels in normal cells triggers a gene expression program. This program mimics features seen in tumor cells, impacting cell cycle and stress responses.
Area of Science:
- Cell Biology
- Genetics
- Cancer Research
Background:
- The centromere is crucial for chromosome segregation during mitosis.
- Kinetochore assembly and microtubule attachments are vital for chromosome stability.
- Aneuploidy, common in cancer, arises from errors in chromosome segregation.
Purpose of the Study:
- To investigate the gene network associated with CENP-E reduction and aneuploidy.
- To explore the gene expression profile triggered by CENP-E reduction in normal cells.
Main Methods:
- siRNA-induced 50% reduction of CENP-E.
- Gene expression microarray analyses at early and late timepoints post-transfection.
Main Results:
- Initially, cell cycle regulation and stress response pathways were downregulated.
- Later, pathways involved in epithelial-mesenchymal transition, hypoxia, and xenobiotic metabolism were altered.
- The study identified a gene network linked to CENP-E reduction and aneuploidy.
Conclusions:
- CENP-E reduction in normal cells induces a gene expression program.
- This program shares characteristics with gene expression profiles found in tumor cells.
- Findings suggest a link between CENP-E function and cancer-related gene expression.
