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cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes.

Daniela-Alejandra Medina-Chávez1, Irene Sánchez-Ajofrín1, Patricia Peris-Frau1

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Summary

Modulating cAMP levels with forskolin and IBMX before in vitro maturation (pre-IVM) in sheep oocytes significantly reduces DNA damage and enhances blastocyst development rates, improving oocyte developmental competence.

Keywords:
DNA damageIBMXcAMPembryoforskolinin vitro maturationoocytesheep

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Area of Science:

  • Reproductive Biology
  • Cellular Biology
  • Biochemistry

Background:

  • The mechanisms of cAMP modulators in improving oocyte developmental competence during in vitro maturation (IVM) are not fully understood.
  • Oocytes are susceptible to DNA damage during in vitro culture, impacting their developmental potential.

Purpose of the Study:

  • To investigate the effects of pre-IVM treatment with cAMP modulators (forskolin and IBMX) on sheep oocyte maturation.
  • To assess the impact of pre-IVM cAMP modulation on nuclear and cytoplasmic maturation, cumulus cell activity, and developmental competence.

Main Methods:

  • Sheep oocytes were treated with 3-isobutyl-1-methylxanthine (IBMX) and forskolin for 2 hours before standard IVM (pre-IVM).
  • Evaluated DNA damage, nuclear and cytoplasmic maturation, cumulus cell activity, and in vitro developmental potential (blastocyst rates).

Main Results:

  • Pre-IVM treatment significantly decreased DNA damage in mature oocytes (2.08% vs. 20.58%).
  • Expanded blastocyst rates were significantly increased by pre-IVM treatment (23.89% vs. 18.22% from total oocytes; 45.16% vs. 32.88% from cleaved embryos).

Conclusions:

  • Pre-IVM modulation of intra-oocyte cAMP levels enhances oocyte DNA repair mechanisms, overcoming in vitro culture-induced damage.
  • This approach improves oocyte developmental competence and offers potential advancements for assisted reproduction technologies.