Characterisation of Bacterial Isolates from Infected Post-Operative Patients in a Malaysian Tertiary Heart Care

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Yi Keng Yong1, Nicole Ce Mun Wen1, Genieve Ee Chia Yeo1

  • 1School of Health Science, International Medical University, Kuala Lumpur 57000, Malaysia.

International Journal of Environmental Research and Public Health
|September 28, 2021
PubMed

Insights

This study investigated bacterial virulence in Malaysian hospital patients, finding common multidrug-resistant species like Klebsiella pneumoniae and Pseudomonas aeruginosa. Some bacteria showed biofilm formation and silenced antibiotic resistance genes, suggesting survival under stress.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Hospital-Acquired Infections

Background:

  • Post-operative infections pose significant risks to hospital patients.
  • Understanding bacterial virulence factors is crucial for effective treatment and prevention.

Purpose of the Study:

  • To explore virulence properties of bacteria causing post-operative infections at the National Heart Institute (IJN) in Malaysia.
  • To investigate antibiotic resistance gene patterns and biofilm formation in bacterial isolates.

Main Methods:

  • Analysis of 127 bacterial isolates from post-operative patients.
  • Investigation of antibiotic resistance genes, including mecA and ermA.
  • Biofilm formation assays.

Main Results:

  • Klebsiella pneumoniae (36.2%), Pseudomonas aeruginosa (26%), and Staphylococcus aureus (23.6%) were the most prevalent isolates.
  • 49 isolates exhibited multidrug resistance genes; mecA and ermA genes were found in susceptible S. aureus strains.
  • 44 isolates demonstrated biofilm production, indicating potential survival mechanisms.

Conclusions:

  • Bacterial isolates from post-operative infections display diverse virulence traits, including multidrug resistance and biofilm formation.
  • The presence of resistance genes in susceptible strains suggests complex resistance mechanisms.
  • Findings highlight the need for enhanced infection control strategies in healthcare settings.
Keywords:
ESBL genesbacterial infectionpostoperative patients

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